Bock K W, Lilienblum W, von Bahr C
Drug Metab Dispos. 1984 Jan-Feb;12(1):93-7.
Human liver samples from a "liver bank" which have been previously characterized by the ability to catalyze cytochrome P-450 dependent reactions were analyzed for various UDP-glucuronyltransferase activities. When stored at -80 degrees C, UDP-glucuronyltransferase activities and latency characteristics of the enzyme appeared to be stable for up to 2 years. The correlation of UDP-glucuronyltransferase activity (4-methylumbelliferone as substrate) with enzyme activities towards 4-nitrophenol and 1-naphthol was much higher (r greater than 0.8) than that (r less than 0.3) observed with other enzyme activities (4-hydroxybiphenyl, morphine, and chloramphenicol as substrates), suggesting the presence of multiple enzyme forms in human liver. Livers of three patients treated with phenytoin or pentobarbital showed significantly higher UDP-glucuronyltransferase activities towards 1-naphthol, 4-methylumbelliferone, and bilirubin than those from five patients who did not receive these inducing agents.
对一个“肝脏库”中先前已通过催化细胞色素P - 450依赖性反应的能力进行表征的人类肝脏样本，分析了其各种UDP - 葡萄糖醛酸基转移酶活性。当保存在-80℃时，UDP - 葡萄糖醛酸基转移酶活性和该酶的潜伏特性在长达2年的时间内似乎都是稳定的。以4 - 甲基伞形酮为底物时UDP - 葡萄糖醛酸基转移酶活性与对4 - 硝基苯酚和1 - 萘酚的酶活性之间的相关性（r大于0.8）远高于以其他酶活性（以4 - 羟基联苯、吗啡和氯霉素为底物）观察到的相关性（r小于0.3），这表明人类肝脏中存在多种酶形式。三名接受苯妥英或戊巴比妥治疗的患者的肝脏对1 - 萘酚、4 - 甲基伞形酮和胆红素的UDP - 葡萄糖醛酸基转移酶活性明显高于五名未接受这些诱导剂的患者的肝脏。
