Bansal S K, Li H C, Holmes G, Gessner T
Res Commun Chem Pathol Pharmacol. 1982 Feb;35(2):291-302.
Fractionation of sodium cholate solubilized microsomes from mouse liver on Sepharose CL-48 yielded three protein peaks with UDP-glucuronyltransferase activities. Of these three peaks, only peak II contained activities towards all the substrates tested: p-nitrophenol, 1-naphthol, morphine, testosterone and estrone. These glucuronyltransferase activities could not be dissociated by further chromatography on DEAE-Sepharose CL-6B and isoelectric focusing. The results show the presence of a functional form of glucuronyltransferase with a wide substrate specificity, and indicate that in addition, other forms with narrower studied specificities may also be present in mouse liver microsomes.
用琼脂糖CL - 48对来自小鼠肝脏的胆酸钠增溶微粒体进行分级分离，得到了三个具有UDP - 葡萄糖醛酸基转移酶活性的蛋白峰。在这三个峰中，只有峰II对所有测试底物：对硝基苯酚、1 - 萘酚、吗啡、睾酮和雌酮都有活性。通过在DEAE - 琼脂糖CL - 6B上进一步层析和等电聚焦，这些葡萄糖醛酸基转移酶活性不能被解离。结果表明存在一种具有广泛底物特异性的葡萄糖醛酸基转移酶功能形式，并且还表明，在小鼠肝脏微粒体中可能还存在其他具有较窄研究特异性的形式。
