Morris R J, Beech J N
Dev Biol. 1984 Mar;102(1):32-42. doi: 10.1016/0012-1606(84)90172-6.
The glycoprotein Thy-1 is found on the surface of both neurons, and most fibroblasts, in tissue culture of embryonic or neonatal rat nervous tissue. In adult rat nerves, however, we find the antigen restricted in vivo to neurons and their axons (R. Morris, P. Barber, J. Beech, and G. Raisman, 1983, J. Neurocytol., 12, 1017-1039). We show here that this discrepancy is due to a loss of Thy-1 from neural connective tissue during postnatal development. Moreover, the different elements of connective tissue loose Thy-1 at different times. Epineurial fibroblasts, for instance, express Thy-1 for at least 2 weeks after endoneurial fibroblasts lack detectable antigen. As judged by their loss of Thy-1 antigen, therefore, the different components of neural connective tissue mature at different rates. One important practical implication of this is that Thy-1 cannot be used as a cell surface "marker" for all classes of fibroblasts in vitro. Unusual problems encountered in the immunohistochemical staining of Thy-1 on perineurium, but not on the other elements of the nerve, are also described.
在胚胎或新生大鼠神经组织的组织培养中，糖蛋白Thy-1存在于神经元和大多数成纤维细胞的表面。然而，在成年大鼠神经中，我们发现该抗原在体内仅限于神经元及其轴突（R. 莫里斯、P. 巴伯、J. 比奇和G. 雷斯曼，1983年，《神经细胞学报》，12卷，第1017 - 1039页）。我们在此表明，这种差异是由于出生后发育过程中神经结缔组织中Thy-1的丢失所致。此外，结缔组织的不同成分在不同时间丢失Thy-1。例如，神经外膜成纤维细胞在神经内膜成纤维细胞缺乏可检测到的抗原后至少2周仍表达Thy-1。因此，根据它们Thy-1抗原的丢失情况判断，神经结缔组织的不同成分以不同速率成熟。这一现象的一个重要实际意义是，Thy-1不能在体外用作所有类型成纤维细胞的细胞表面“标志物”。文中还描述了在神经束膜上进行Thy-1免疫组织化学染色时遇到的异常问题，但在神经的其他成分上未出现此类问题。
