Gunter K C, Malek T R, Shevach E M
J Exp Med. 1984 Mar 1;159(3):716-30. doi: 10.1084/jem.159.3.716.
We have identified a single rat monoclonal antibody, G7, that is a potent inducer of interleukin (IL-2) production from all functioning T cell hybridomas as well as from normal T cells. G7 is also mitogenic for normal T cells and is a very effective inducer of IL-2 receptor expression. On fluorescence-activated cell sorter analysis, G7 recognized a pan-T cell antigen. Immunoprecipitation studies demonstrated that G7 recognized a cell surface molecule of 28-32 kD that appeared to be identical to Thy-1 in coprecipitation studies. In addition, G7 precipitated a protein of 50 kD. The possible relationship of the putative molecular complex identified by G7 on murine cells to the molecular complex identified on human T cells with anti-T3 reagents is discussed. In addition, G7 should prove to be a very useful reagent for studying the early events of lymphocyte activation as well as an inducer of lymphokine-rich supernatants.
我们已鉴定出一种单一的大鼠单克隆抗体G7,它是所有功能性T细胞杂交瘤以及正常T细胞产生白细胞介素(IL-2)的强效诱导剂。G7对正常T细胞也有促有丝分裂作用,并且是IL-2受体表达的非常有效的诱导剂。在荧光激活细胞分选分析中,G7识别一种泛T细胞抗原。免疫沉淀研究表明,G7识别一种28 - 32 kD的细胞表面分子,在共沉淀研究中该分子似乎与Thy-1相同。此外,G7沉淀出一种50 kD的蛋白质。讨论了G7在鼠细胞上鉴定出的假定分子复合物与用抗T3试剂在人T细胞上鉴定出的分子复合物之间的可能关系。此外,G7应被证明是研究淋巴细胞激活早期事件以及富含淋巴因子的上清液诱导剂的非常有用的试剂。