Wittmann J, Kugler W, Rahn H
Comp Biochem Physiol A Comp Physiol. 1984;77(3):547-51. doi: 10.1016/0300-9629(84)90226-3.
Treating chick embryos with high doses of thiourea (32.8 mumol) on day 17 of incubation resulted in prevention of hatching and of active breathing. Furthermore, thiourea also prevented the increase of O2 consumption and the marked increase of somatic activity associated with the final hatching act. These findings provide evidence for the importance of active breathing in the prenatal period to initiate pipping and hatching of the avian embryo.
在孵化第17天用高剂量硫脲（32.8微摩尔）处理鸡胚，可导致孵化和主动呼吸受到抑制。此外，硫脲还可抑制氧气消耗的增加以及与最终孵化行为相关的躯体活动的显著增加。这些发现为产前主动呼吸对启动鸟类胚胎啄壳和孵化的重要性提供了证据。
