使用脱氧核糖核酸酶制备不含宿主组织DNA的立克次氏体悬浮液。
The use of DNase for preparation of host tissue DNA-free rickettsial suspensions.
作者信息
Ogarkova O A, Balayeva N M
出版信息
Acta Virol. 1984 Jan;28(1):73-7.
PMID:6143501
Abstract
Treatment of Rickettsia prowazekii (strain Breinl) grown in chick embryo (CE) yolk sacs with DNase resulted in preparation of host tissue DNA-free rickettsial suspensions suitable for isolation of rickettsial DNA. Of two DNA fractions obtained, the first and main one corresponded to rickettsial DNA (29% G + C) based on the physico-chemical characteristics, whereas the nature of second supplementary fraction (47.5% G + C) was not determined.
摘要
用脱氧核糖核酸酶（DNase）处理在鸡胚（CE）卵黄囊中生长的普氏立克次体（布雷因尔菌株），得到了不含宿主组织DNA的立克次体悬浮液，适用于立克次体DNA的分离。在获得的两个DNA组分中，根据物理化学特性，第一个也是主要的组分对应于立克次体DNA（G + C含量为29%），而第二个补充组分（G + C含量为47.5%）的性质尚未确定。
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