Hand A R, Oliver C
J Cell Biol. 1977 Aug;74(2):399-413. doi: 10.1083/jcb.74.2.399.
The method of secretory granuleformation in the acinar cells of the rat exorbital lacrimal gland was studied by electron microscope morphological and cytochemical techniques. Immature secretory granules at the inner face of the Golgi apparatus were frequently attached to a narrow cisternal structure similar to GERL as described in neurons by Novikoff et al. (Novikoff, P. M., A. B. Novikoff, N. Quintana, and J.-J. Hauw. 1971. J. Cell Bio. 50:859-886). In the lacrimal gland. GERL was located adjacent to the inner Golgi saccule, or separated from it by a variable distance. Portions of GERL were often closely paralleled by modified cisternae of rough endoplasmic reticulum (RER), which lacked ribosomes on the surface adjacent to GERL. Diaminobenzidine reaction product of the secretory enzyme peroxidase was localized in the cisternae of the nuclear envelope, RER, peripheral Golgi vesicles, Golgi saccules, and immature and mature secretory granules. GERL was usually free of peroxidase reaction product or contained only a small amount. Thiamine pyrophosphatase reaction product was present in two to four inner Golgi saccules; occasionally, the innermost saccule was dilated and fenestrated, and contained less reaction product than the next adjacent saccule. Acid phosphatase (AcPase) reaction product was present in GERL, immature granules, and, rarely, in the innermost saccule, but not in the rest of the Golgi saccules. Thick sections of AcPase preparations viewed at 100 kV revealed that GERL consisted of cisternal, and fenestrated or tublular portions. The immature granules were attached to GERL by multiple connections to the tublular portions. These results suggest that, in the rat exorbital lacrimal gland, the Golgi saccules participate in the transport of secretory proteins, and that GERL is involved in the formation of secretory granules.
采用电子显微镜形态学和细胞化学技术，研究了大鼠眶外泪腺腺泡细胞中分泌颗粒的形成方法。高尔基体内侧的未成熟分泌颗粒经常附着于一种狭窄的池状结构，类似于诺维科夫等人在神经元中描述的GERL（诺维科夫，P.M.，A.B.诺维科夫，N.金塔纳，和J.-J.豪。1971年。《细胞生物学杂志》50:859 - 886）。在泪腺中，GERL位于高尔基体内侧囊泡附近，或与之相隔一定距离。GERL的部分区域常与糙面内质网（RER）的修饰池紧密平行，在靠近GERL的表面缺乏核糖体。分泌酶过氧化物酶的二氨基联苯胺反应产物定位于核膜池、RER、外周高尔基体小泡、高尔基体囊泡以及未成熟和成熟的分泌颗粒中。GERL通常没有过氧化物酶反应产物或仅含有少量。硫胺素焦磷酸酶反应产物存在于两到四个高尔基体内侧囊泡中；偶尔，最内侧的囊泡会扩张并有小孔，且所含反应产物比相邻的下一个囊泡少。酸性磷酸酶（AcPase）反应产物存在于GERL、未成熟颗粒中，很少存在于最内侧的囊泡中，但不存在于高尔基体其他囊泡中。在100 kV下观察的AcPase制剂厚切片显示，GERL由池状、有小孔或管状部分组成。未成熟颗粒通过与管状部分的多个连接附着于GERL。这些结果表明，在大鼠眶外泪腺中，高尔基体囊泡参与分泌蛋白的运输，而GERL参与分泌颗粒的形成。
