Hand A R, Oliver C
J Histochem Cytochem. 1984 Apr;32(4):403-12. doi: 10.1177/32.4.6142913.
The structure and cytochemistry of the Golgi apparatus and GERL of rat parotid acinar cells was studied after in vivo secretory stimulation with isoproterenol. Discharge of mature secretory granules was complete within 1 hr after isoproterenol injection, but immature granules in the Golgi region or near the lumen were not released. At early times (1-5 hr) after isoproterenol, acid phosphatase (AcPase) activity was markedly increased in GERL and immature secretory granules compared to uninjected controls. GERL appeared increased in extent and numerous continuities with immature granules were observed. Reaccumulation of mature secretory granules was first evident at 5 hr, and was almost complete by 16 hr after isoproterenol. Thiamine pyrophosphatase (TPPase) activity, normally restricted to the trans Golgi saccules, was frequently present in immature granules during this time. Narrow cisternae resembling GERL, occasionally in continuity with immature granules, also contained TPPase reaction product. By 16-24 hr after stimulation, the activity and distribution of AcPase and TPPase were similar to control cells. These results demonstrate the dynamic nature of the Golgi apparatus and GERL in parotid acinar cells, and emphasize the close structural and functional relationship between these two structures.
用异丙肾上腺素对大鼠腮腺腺泡细胞进行体内分泌刺激后，研究了其高尔基体和GERL的结构及细胞化学。异丙肾上腺素注射后1小时内，成熟分泌颗粒的排放完成，但高尔基体区域或靠近管腔的未成熟颗粒未被释放。在异丙肾上腺素作用后的早期（1 - 5小时），与未注射的对照组相比，GERL和未成熟分泌颗粒中的酸性磷酸酶（AcPase）活性显著增加。GERL的范围似乎增大，并且观察到与未成熟颗粒有许多连续性。成熟分泌颗粒的重新积累在5小时时首次明显，异丙肾上腺素作用后16小时时几乎完成。硫胺焦磷酸酶（TPPase）活性通常局限于高尔基体反面膜囊，在此期间经常出现在未成熟颗粒中。类似于GERL的狭窄池，偶尔与未成熟颗粒连续，也含有TPPase反应产物。刺激后16 - 24小时，AcPase和TPPase的活性和分布与对照细胞相似。这些结果证明了腮腺腺泡细胞中高尔基体和GERL的动态性质，并强调了这两种结构之间密切的结构和功能关系。
