McGinty J F, van der Kooy D, Bloom F E
J Neurosci. 1984 Apr;4(4):1104-17. doi: 10.1523/JNEUROSCI.04-04-01104.1984.
Pharmacological and biochemical evidence has implied that a widespread opioid peptide system exists within the cerebral cortex to mediate a variety of opiate effects. However, immunocytochemical detection of opioid peptides in the cortex has been limited. Using antisera to enkephalin and bovine adrenal medullary peptide, both fragments of proenkephalin, and an antiserum to dynorphin A, a fragment of prodynorphin, we now describe the regional and laminar distribution of a widespread population of olfactory cortical and neocortical cell bodies and fibers with opioid immunoreactivity in rats. Neurons stained with each antiserum are distributed bimodally in layers II and III and V and VI of neocortex as well as in layers II and III of olfactory cortex. The widespread distribution and heterogeneous morphology of cortical cells containing proenkephalin and dynorphin-A immunoreactivity suggest that opioid peptide-containing neurons may influence the functioning of local, commissural, and projection neurons in rat cerebral cortex.
药理学和生物化学证据表明，大脑皮质中存在广泛的阿片肽系统，以介导多种阿片类药物的作用。然而，皮质中阿片肽的免疫细胞化学检测一直很有限。利用针对脑啡肽和牛肾上腺髓质肽（二者均为前脑啡肽的片段）的抗血清，以及针对强啡肽A（前强啡肽的片段）的抗血清，我们现在描述了大鼠嗅觉皮质和新皮质中具有阿片免疫反应性的广泛细胞体和纤维群体的区域和层状分布。用每种抗血清染色的神经元在新皮质的II层和III层、V层和VI层以及嗅觉皮质的II层和III层呈双峰分布。含有前脑啡肽和强啡肽A免疫反应性的皮质细胞的广泛分布和异质形态表明，含阿片肽的神经元可能影响大鼠大脑皮质中局部、连合和投射神经元的功能。
