Jew J Y, Léránth C, Arimura A, Palkovits M
Neuroendocrinology. 1984 Mar;38(3):169-75. doi: 10.1159/000123886.
Glass microknife lesions and immunocytochemistry were used to evaluate luteinizing hormone-releasing hormone (LH-RH)- and somatostatin (SS)-immunoreactive pathways from the preoptic region to the rat median eminence. Cuts were so placed that axons of more caudally located neurons in the periventricular hypothalamic areas were spared. Light and EM observations of LH-RH-immunostained preparations indicated that following the midline periventricular cuts the density of LH-RH labelled axons and axon terminals in the ME appeared similar to that of nonlesioned animals. Following bilateral lateral hypothalamic cuts placed between the preoptic area and the ME, LH-RH immunostaining in the ME was markedly reduced. This provides evidence that the preponderance of LH-RH axons originating from the preoptic area reach the ME by a lateral hypothalamic route. In contrast to the LH-RH findings, midline lesions made using the same coordinates caused a noticeable reduction in SS immunostaining in the accurate nucleus and ME. There was either no change or only minimal change after the lateral cut. Somatostatin axons arising from the preoptic periventricular nucleus take a periventricular route and contribute to median eminence innervation, but much less extensively than the more caudally located somatostatin neurons in the hypothalamic periventricular nucleus [19].
使用玻璃微刀损伤和免疫细胞化学方法，评估从视前区到大鼠正中隆起的促黄体生成激素释放激素（LH-RH）和生长抑素（SS）免疫反应性通路。切口位置的设置使得下丘脑室周区域中更靠尾侧的神经元轴突得以保留。对LH-RH免疫染色标本的光镜和电镜观察表明，在进行脑室周中线切断后，正中隆起中LH-RH标记的轴突和轴突终末的密度与未损伤动物相似。在视前区和正中隆起之间进行双侧下丘脑外侧切断后，正中隆起中的LH-RH免疫染色明显减少。这提供了证据，表明源自视前区的LH-RH轴突主要通过下丘脑外侧途径到达正中隆起。与LH-RH的结果相反，使用相同坐标进行的中线损伤导致弓状核和正中隆起中SS免疫染色明显减少。外侧切断后要么没有变化，要么只有微小变化。源自视前室周核的生长抑素轴突走脑室周途径，并参与正中隆起的神经支配，但程度远低于下丘脑室周核中更靠尾侧的生长抑素神经元[19]。
