Naik D V
Cell Tissue Res. 1976 Oct 6;173(2):143-66. doi: 10.1007/BF00221371.
Immunohistochemical localization of luteinizing hormone-releasing hormone (LH-RH), during different phases of the estrus cycle, in the preoptic, suprachiasmatic and arcuate nuclei, and in the OVLT of rats, with special emphasis on the ependymal cells, was studied by light, fluorescent and electron microscopy, by using rabbit anti serum to synthetic LH-RH. The LH-RH neurons in the above mentioned areas, were very active during late diestrus and early proestrus phases. Specialized ependymal cells bordering the 3rd ventricle also showed varied LH-RH positive reaction during different phases of the estrus cycle. Immunofluorescent studies showed cyclic variations in the LH-RH material in the CSF of the preoptic and infundibular recesses, as well as in the 3rd ventricle near OVLT, in that, it was maximum during late diestrus and early proestrus phases. Immediately after this, the LH-RH late proestrus was reached. We have also observed that during the proestrus phase, as the LH-RH material started declining in the CSF, it had started building up in the specialized ependyma. Estrus, metaestrus and early diestrus phases showed very weak immunofluorescent LH-RH material in the lumen of the infundibular recess and in the specialized ependyma. Our immuno-electron microscopic observations showed pleomorphic LH-RH granules in the specialized ependyma during late kiestrus and proestrus phases. All these observations lead us to believe that LH-RH is not synthesized in the ependymal cells,but is phagocytosed from the CSF of the 3rd ventricle by the specialized ependyma, which transports it to the ME portal system. In males, the fluorescent LH-RH material did not show any noticeable changes. With the present and previous work,it is concluded that the neurons in differentnuclei synthesize LH-RH and transport it to the ME portal system,primarily through the nerve fibers and secondarily by the ventricular route. It is also suggested that the ependymal transport of LH-RH to the ME portal system is cyclic and thus controls the gonadotropin secretion.
利用兔抗合成促黄体生成激素释放激素（LH - RH）血清，通过光学显微镜、荧光显微镜和电子显微镜，研究了发情周期不同阶段大鼠视前核、视交叉上核、弓状核以及终板血管器（OVLT）中LH - RH的免疫组织化学定位，特别关注室管膜细胞。上述区域的LH - RH神经元在动情后期和动情前期非常活跃。与第三脑室相邻的特化室管膜细胞在发情周期的不同阶段也表现出不同的LH - RH阳性反应。免疫荧光研究显示，视前区和漏斗隐窝脑脊液中以及OVLT附近第三脑室中的LH - RH物质呈周期性变化，即在动情后期和动情前期含量最高。此后不久，达到LH - RH的动情前期晚期。我们还观察到，在动情前期，随着脑脊液中LH - RH物质开始减少，它已开始在特化室管膜中积累。发情期、间情期和动情前期早期，漏斗隐窝管腔和特化室管膜中的免疫荧光LH - RH物质非常弱。我们的免疫电子显微镜观察显示，在动情后期和动情前期，特化室管膜中有多形性LH - RH颗粒。所有这些观察结果使我们相信，LH - RH不是在室管膜细胞中合成的，而是由特化室管膜从第三脑室的脑脊液中吞噬，然后将其转运至正中隆起门静脉系统。在雄性中，荧光LH - RH物质未显示任何明显变化。结合目前和以往的研究工作得出结论，不同核团中的神经元合成LH - RH并将其转运至正中隆起门静脉系统，主要通过神经纤维，其次通过脑室途径。还表明LH - RH向正中隆起门静脉系统的室管膜转运是周期性的，从而控制促性腺激素的分泌。
