Ueno M, Mizuhira V
Histochemistry. 1984;80(3):213-7. doi: 10.1007/BF00495768.
Periodical changes in Ca2+-ATPase and Mg2+-ATPase activity were observed cytochemically in the crayfish gastrolith epithelium during the molting cycle in relation to the calcium transport mechanism. The ATPase activity was demonstrated by a new one-step lead citrate method. The reaction products were mainly restricted to the matrix of type II cell mitochondria. The Ca2+-ATPase activity was intensely observed in two calcium moving stages, the small gastrolith period which indicates the beginning of gastrolith formation, and the aftermolt , when the calcified gastrolith has been dissolved in the stomach and then reabsorbed from the stomach epithelium into the newly formed soft exoskeleton through the blood. Although the intensity of reaction products of Mg2+-ATPase varied in each stage, the enzymatic activity was observed throughout all molting stages. Reaction products were observed in all mitochondria, basement membranes, apical cytoplasmic membranes, and in some lysosomes. In conclusion, periodical changes in the two types of ATPase activity were seen in the mitochondria of gastrolith epithelium during the molting cycle, but Ca2+-ATPase activity seemed to be more prominently synchronized to the calcium movement in the gastrolith epithelium than Mg2+-ATPase activity. There results provide the strong evidence that Ca2+-ATPase may act strongly in the calcium transport system of crayfish molting.
在小龙虾蜕壳周期中，通过细胞化学方法观察到与钙转运机制相关的胃石上皮中Ca2 + -ATP酶和Mg2 + -ATP酶活性的周期性变化。ATP酶活性通过一种新的一步柠檬酸铅法进行检测。反应产物主要局限于II型细胞线粒体基质中。在两个钙移动阶段强烈观察到Ca2 + -ATP酶活性，即表明胃石形成开始的小胃石期，以及蜕壳后，此时钙化的胃石已在胃中溶解，然后通过血液从胃上皮重新吸收到新形成的软外骨骼中。尽管Mg2 + -ATP酶反应产物的强度在每个阶段有所不同，但在所有蜕壳阶段均观察到酶活性。在所有线粒体、基底膜、顶端细胞质膜以及一些溶酶体中均观察到反应产物。总之，在蜕壳周期中，胃石上皮线粒体中观察到两种类型ATP酶活性的周期性变化，但Ca2 + -ATP酶活性似乎比Mg2 + -ATP酶活性更明显地与胃石上皮中的钙移动同步。这些结果提供了有力证据，表明Ca2 + -ATP酶可能在小龙虾蜕壳的钙转运系统中发挥重要作用。
