豚鼠中耳黏膜中Ca2(+)-ATP酶活性的超细胞化学定位
Ultracytochemical localization of Ca2(+)-ATPase activity in the middle ear mucosa of the guinea pig.
作者信息
Karatay M C, Mann W, Beck C, Vit M
机构信息
ENT Department, University of Freiburg, Federal Republic of Germany.
出版信息
Arch Otorhinolaryngol. 1989;246(6):423-7. doi: 10.1007/BF00464302.
Ca2(+)-ATPase activity was studied ultra-cytochemically in the middle ear mucosa of the guinea pig. On electron microscopic examination, the most intense reaction was found on the microvilli. Reaction products were also observed on the cilia and around and between the secretory granules on the apical side of the cells in their secretory phase. The basolateral membranes contained few reaction products, while very little or no activity was found on the basal membrane.
采用超微细胞化学方法研究了豚鼠中耳黏膜中Ca2(+)-ATP酶活性。电镜检查发现，微绒毛上的反应最为强烈。在纤毛上以及处于分泌期的细胞顶端的分泌颗粒周围和之间也观察到反应产物。基底外侧膜上的反应产物较少，而基底膜上的活性非常低或未检测到活性。
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