法医相关样本中苯甲酰爱康宁的放射免疫分析。

Radioimmunoassay of benzoylecgonine in samples of forensic interest.

作者信息

Robinson K, Smith R N

出版信息

J Pharm Pharmacol. 1984 Mar;36(3):157-62. doi: 10.1111/j.2042-7158.1984.tb06930.x.

DOI:10.1111/j.2042-7158.1984.tb06930.x

Abstract

A simple and economical radioimmunoassay for benzoylecgonine in blood or urine is described. Haemolysis, decomposition, common anticoagulants and sodium fluoride do not affect the results. A commercially available antiserum is used at a dilution of 1:600. The tracer is radioiodinated p-hydroxybenzoylecgonine. It is prepared by reacting p-acetoxybenzoic anhydride with methyl ecgonine followed by mild hydrolysis and radioiodination by the Iodo-gen method. The product is purified on a disposable silica cartridge and has a specific activity of about 30 TBq mmol-1. Polyethylene glycol is used to separate the bound and free fractions in the assay. The range of the dose-response curve is 0-400 ng ml-1 benzoylecgonine. The assay is largely specific for benzoylecgonine. Cocaine, ecgonine, methylecgonine and cinnamoyl cocaine have slight or negligible cross-reactivities. The inter-assay coefficient of variation is 7.5% and the recovery of benzoylecgonine from 'spiked' blood is 103%. The 'cut-off' is 20 ng ml-1 benzoylecgonine for both blood and urine. Radioimmunoassay and high-performance liquid chromatography results agree well.

摘要

本文描述了一种用于检测血液或尿液中苯甲酰芽子碱的简单且经济的放射免疫分析方法。溶血、分解、常见抗凝剂和氟化钠均不影响检测结果。使用市售抗血清，稀释比例为1:600。示踪剂为放射性碘标记的对羟基苯甲酰芽子碱。它通过对乙酰氧基苯甲酸酐与甲基芽子碱反应，随后进行温和水解，并采用碘代法进行放射性碘化制备而成。产物在一次性硅胶柱上进行纯化，比活度约为30 TBq mmol-1。在分析中使用聚乙二醇分离结合部分和游离部分。剂量反应曲线范围为0 - 400 ng ml-1苯甲酰芽子碱。该分析方法对苯甲酰芽子碱具有较高的特异性。可卡因、芽子碱、甲基芽子碱和肉桂酰可卡因具有轻微或可忽略不计的交叉反应性。批间变异系数为7.5%，从“加标”血液中回收苯甲酰芽子碱的回收率为103%。血液和尿液中苯甲酰芽子碱的“截断值”均为20 ng ml-1。放射免疫分析结果与高效液相色谱结果吻合良好。