Takahashi K, Isobe T, Ohtsuki Y, Akagi T, Sonobe H, Okuyama T
Virchows Arch B Cell Pathol Incl Mol Pathol. 1984;45(4):385-96. doi: 10.1007/BF02889881.
The immunohistochemical distribution and localization of the alpha and beta subunits of S-100 protein in human neoplasms and normal tissues were studied by the PAP method using monospecific rabbit antibodies against each subunit. Beta subunit immunoreactivity was detected in all S-100-positive cells and tumors reported previously. In contrast alpha subunit immunoreactivity was absent from Schwann cells, schwannomas, neurofibromas, granular cell myoblastomas, pituicytes of the neurohypophysis, Langerhans cells, interdigitating reticulum cells, and histiocytosis X cells. Interestingly, only the alpha subunit was detected in neurons of both central and peripheral nervous system, and in lymph node macrophages. Human S-100-positive cells are divided into three groups; the first is composed of cells containing only the beta subunit (probably S-100b; beta beta), the second consists of cells containing both the alpha and beta subunits, and the third is composed of cells containing only the alpha subunit (probably S- 100ao ; alpha alpha). The ontogentic relationships between S-100-positive cells and tumors are discussed in the light of these findings.
采用抗各亚基的单特异性兔抗体，运用PAP法研究了S - 100蛋白α和β亚基在人类肿瘤组织和正常组织中的免疫组化分布及定位。在先前报道的所有S - 100阳性细胞和肿瘤中均检测到β亚基免疫反应性。相比之下，施万细胞、神经鞘瘤、神经纤维瘤、颗粒细胞成肌细胞瘤、神经垂体的垂体细胞、朗格汉斯细胞、交错突网状细胞和组织细胞增生症X细胞中未检测到α亚基免疫反应性。有趣的是，仅在中枢神经系统和周围神经系统的神经元以及淋巴结巨噬细胞中检测到α亚基。人类S - 100阳性细胞分为三组：第一组由仅含β亚基的细胞组成（可能是S - 100b；ββ），第二组由同时含α和β亚基的细胞组成，第三组由仅含α亚基的细胞组成（可能是S - 100αo；αα）。根据这些发现讨论了S - 100阳性细胞与肿瘤之间的个体发生关系。
