Mori M, Takai Y, Naito R, Hosaka M, Murase N
Virchows Arch B Cell Pathol Incl Mol Pathol. 1984;45(4):431-41. doi: 10.1007/BF02889884.
Immunohistochemical demonstration of epidermal growth factor (EGF) and nerve growth factor (NGF) was made during chemical carcinogenesis in the mouse submandibular gland. The granular convoluted tubule cells in the normal male submandibular gland contained larger amounts of EGF and NGF than in the female. The initial phase and early stages in chemical carcinogenesis showed degranulation of the granular convoluted tubule cells with a marked decrease in EGF and NGF. Premalignant lesions such as duct-like structures and multicystic lesions showed variable staining for EGF and were usually negative for NGF. Material secreted into the luminal spaces revealed increased staining for EGF and NGF. Scattered tumor cells of the poorly differentiated squamous-cell carcinoma type and desquamated tumor cells contained abundant EGF, but not NGF. No positive reaction for EGF or NGF was found in the induced squamous-cell carcinoma cells.
在小鼠下颌下腺化学致癌过程中进行了表皮生长因子（EGF）和神经生长因子（NGF）的免疫组织化学检测。正常雄性下颌下腺的颗粒曲管细胞所含的EGF和NGF比雌性的多。化学致癌的初始阶段和早期显示颗粒曲管细胞脱颗粒，EGF和NGF显著减少。癌前病变如导管样结构和多囊性病变对EGF呈可变染色，对NGF通常呈阴性。分泌到管腔间隙的物质显示EGF和NGF染色增加。低分化鳞状细胞癌类型的散在肿瘤细胞和脱落的肿瘤细胞含有丰富的EGF，但不含NGF。在诱导的鳞状细胞癌细胞中未发现EGF或NGF的阳性反应。
