Takai Y, Hyun K H, Hosaka M, Murase N, Mori M
J Oral Pathol. 1984 Aug;13(4):429-37. doi: 10.1111/j.1600-0714.1984.tb01443.x.
The lectin, Concanavalin A(Con A) has been used to localize specific sugar residues (D-glucose, D-mannose and D-fructose) in premalignant lesions and squamous-cell carcinomas induced following cryosurgery of the mouse submandibular gland. The original Con A-horseradish peroxidase (HRP) technique as well as its combination with periodate oxidation and subsequent reduction by borohydrate were used to compare the epithelial elements during submandibular gland carcinogenesis. Granules in the granular convoluted tubule cells which were weakly reactive to the Con A-HRP method were not present in the premalignant duct like structures. The epithelium of premalignant lesions, duct-like structures, multicystic lesions, and squamous-cell carcinomas were positive for the cell-surface and intercellular substances; and basement membranes and stromal fibers were also positive. The results indicated that throughout malignant transformation of the ductal segments, premalignant epithelia lost Con A-HRP-staining granules and that Con A-binding patterns in induced squamous-cell carcinomas were similar to those found in squamous-cell epithelium.
凝集素伴刀豆球蛋白A（Con A）已被用于在小鼠下颌下腺冷冻手术后诱导的癌前病变和鳞状细胞癌中定位特定的糖残基（D-葡萄糖、D-甘露糖和D-果糖）。最初的Con A-辣根过氧化物酶（HRP）技术及其与高碘酸盐氧化和随后硼氢化物还原的组合被用于比较下颌下腺癌发生过程中的上皮成分。颗粒曲管细胞中的颗粒对Con A-HRP方法反应较弱，在癌前导管样结构中不存在。癌前病变、导管样结构、多囊性病变和鳞状细胞癌的上皮细胞表面和细胞间物质呈阳性；基底膜和基质纤维也呈阳性。结果表明，在导管段的整个恶性转化过程中，癌前上皮细胞失去了Con A-HRP染色颗粒，并且诱导的鳞状细胞癌中的Con A结合模式与鳞状上皮中的相似。
