Ivanova-Chemishanska L, Izmirova N
Probl Khig. 1977;3:19-24.
35S-cysteine incorporation in the protein synthesis of liver and testes is studied in groups of 20 white rats each, following 14--day poisoning with dithiocarbamates, at doses 1/5 LD50, as follows: group one, cineb - o,180 g/kg, maneb - 0,140 g/kg, and mankozeb - 0,260 g/kg. Protein incorporation in liver and testes is studied after decapitation of the animals, within two hours of intraperitoneal 35S-cysteine injection at dose 15 micro C per rat. Liver and testes undergo appropriate treatment after the method of Munro et al./10/, as modified by Sheitanov /9/, and counting is done with VAZ--310. The results point to a varying degree of cysteine incorporation increase in the protein synthesis of liver and testes. It is attributed to a variety of dystrophic and necrobiotic changes in the tissues of either of the organs, taking place under the effect of dithiocarbamate preparations. An enhancement of regenerative processes in the above mentioned organs, associated with intensified protein synthesis, is by no means ruled out. Differences established in the intensity of amino acid incorporation correlate with the degree of protein synthesis disorders, mostly in terms of the effect of maneb preparations.
每组20只白色大鼠，在以1/5 LD50剂量的二硫代氨基甲酸盐中毒14天后，研究35S-半胱氨酸在肝脏和睾丸蛋白质合成中的掺入情况，如下：第一组，代森锌-0.180克/千克，代森锰-0.140克/千克，和代森锰锌-0.260克/千克。在给动物腹腔注射剂量为每只大鼠15微居里的35S-半胱氨酸后两小时内，将动物断头，然后研究肝脏和睾丸中的蛋白质掺入情况。肝脏和睾丸按照Munro等人[10]的方法进行适当处理，并经Sheitanov[9]修改，使用VAZ-310进行计数。结果表明，肝脏和睾丸蛋白质合成中半胱氨酸掺入量有不同程度的增加。这归因于在二硫代氨基甲酸盐制剂的作用下，任一器官组织中发生的各种营养不良和坏死性变化。上述器官中与蛋白质合成增强相关的再生过程的增强也绝非不可能。所确定的氨基酸掺入强度的差异与蛋白质合成紊乱的程度相关，主要是就代森锰制剂的作用而言。
