Yin X Y, Koshimizu T, Yokota Y, Shibayama K, Ohyama Y, Yashiro K
Department of Pediatrics, Kitasato University, School of Medicine, Sagamihara, Japan.
Acta Paediatr Jpn. 1992 Feb;34(1):6-11. doi: 10.1111/j.1442-200x.1992.tb00917.x.
Ontogenic expression of somatostatin (SRIF) -messenger RNA (mRNA) in the gastrointestinal tract was examined in neonatal rats aged from 1 day preterm to 60 days postpartum in comparison with that in the hypothalamus. SRIF-mRNA in the hypothalamus was already expressed in prenatal rats and its developmental change was relatively small. In contrast, a unique pattern of SRIF-mRNA expression was seen in the different intestinal regions, gastric antrum, duodenum, jejunum and colon. In the duodenum, SRIF-mRNA level was low at birth, markedly increased during the postnatal 3 days and declined to the previous level by day 21. Jejunal SRIF-mRNA was found in neonates but progressively decreased in a similar way to duodenum. On the contrary, gastric SRIF-mRNA level, which was low during early development, rose rapidly to a peak on day 21 and gradually declined to an adult level. In the colon age-related change was not conspicuous, remaining at a low level. These results indicate that (1) expression of SRIF gene in the intestinal tract is regulated by local factor(s) as well as developmental stage, and (2) shift of SRIF-mRNA pattern occurs during weaning from the duodenum-dominant infantile pattern to the gastric-dominant adult pattern.
研究了新生大鼠从早产1天到出生后60天胃肠道中生长抑素（SRIF）-信使核糖核酸（mRNA）的个体发生表达，并与下丘脑进行了比较。下丘脑的SRIF-mRNA在产前大鼠中就已表达，其发育变化相对较小。相比之下，在不同的肠道区域、胃窦、十二指肠、空肠和结肠中观察到SRIF-mRNA表达的独特模式。在十二指肠中，SRIF-mRNA水平在出生时较低，在出生后3天显著增加，并在第21天降至先前水平。空肠SRIF-mRNA在新生儿中被发现，但以与十二指肠类似的方式逐渐减少。相反，胃SRIF-mRNA水平在早期发育时较低，在第21天迅速上升至峰值，然后逐渐降至成年水平。在结肠中，与年龄相关的变化不明显，维持在较低水平。这些结果表明：（1）肠道中SRIF基因的表达受局部因素以及发育阶段的调节；（2）在断奶期间，SRIF-mRNA模式从以十二指肠为主的婴儿模式转变为以胃为主的成年模式。
