Biebricher C K, Düker E M
J Gen Microbiol. 1984 Apr;130(4):941-9. doi: 10.1099/00221287-130-4-941.
Methods for the direct visualization of F and type 1 pili of Escherichia coli in the light microscope are described. The method for visualizing F pili is based on the specific adsorption of fluorescent dye-labelled RNA phages to F pili. The best results were obtained with MS2 phages labelled with rhodamine B. Semi-quantitative determination of the amount of F pili is possible. Type 1 pili can be visualized rapidly and specifically by indirect immunofluorescence. Other structures on the cell surface are neither detected by, nor interfere with these assays. By using different fluorescent dyes the two methods can be combined and both F and type 1 pili can be determined in the same sample.
本文描述了在光学显微镜下直接观察大肠杆菌F菌毛和1型菌毛的方法。观察F菌毛的方法基于荧光染料标记的RNA噬菌体对F菌毛的特异性吸附。用罗丹明B标记的MS2噬菌体可获得最佳结果。F菌毛数量的半定量测定是可行的。1型菌毛可通过间接免疫荧光快速、特异性地观察到。细胞表面的其他结构既不会被这些检测方法检测到，也不会干扰这些检测。通过使用不同的荧光染料，这两种方法可以结合使用，并且可以在同一样本中同时测定F菌毛和1型菌毛。
