Biebricher C K, Düker E M
J Gen Microbiol. 1984 Apr;130(4):951-7. doi: 10.1099/00221287-130-4-951.
F and type 1 piliation of various Escherichia coli K12 Hfr and F+ strains was re-examined by using a new visualization assay. In anaerobic cultures in rich media, bacteria were well F piliated throughout all growth phases. In aerobic cultures in rich media, F piliation reached a maximum in the mid-exponential phase. The yield of pili was up to 15 mg 1(-1). In aerated cultures, F pili disappeared in the late exponential phase. In rich media F pili were on average 10-20 micron long; in synthetic media they were an order of magnitude shorter, and less numerous. Addition of metabolic poisons (NaCN, NaN3, Na3AsO4, phenylethyl alcohol) and starving the bacteria caused rapid disappearance of F pili under aerobic conditions, but had no influence on F piliation under anaerobic conditions. Type 1 piliation was not influenced by these drugs or by an alteration of growth conditions.
通过一种新的可视化检测方法，对各种大肠杆菌K12 Hfr和F+菌株的F和1型菌毛进行了重新检测。在富含培养基的厌氧培养中，细菌在所有生长阶段都有良好的F菌毛。在富含培养基的需氧培养中，F菌毛在指数中期达到最大值。菌毛产量高达15 mg 1(-1)。在通气培养中，F菌毛在指数后期消失。在富含培养基中，F菌毛平均长10 - 20微米；在合成培养基中，它们短一个数量级，且数量较少。添加代谢毒物（NaCN、NaN3、Na3AsO4、苯乙醇）以及使细菌饥饿会导致在需氧条件下F菌毛迅速消失，但在厌氧条件下对F菌毛形成没有影响。1型菌毛不受这些药物或生长条件改变的影响。
