多氯联苯混合物1254处理对杂交太阳鱼体内甲苯、苯胺和氨基比林体外肝脏代谢的影响。

Effects of Aroclor 1254 treatment on the in vitro hepatic metabolism of toluene, aniline and aminopyrine in hybrid sunfish.

作者信息

Carter F D, Puyear R L, Brammer J D

出版信息

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1984;78(1):137-40. doi: 10.1016/0742-8413(84)90060-4.

Abstract

The in vitro metabolism of the volatile aromatic hydrocarbon toluene by enzymes associated with the 12,000 g supernatant fraction of hybrid sunfish (Lepomis macrochirus X L. cyanellus X L. gibbosus) liver homogenates was studied. Aminopyrine demethylase (APDM) and aniline hydroxylase (AH) activities were measured. Intramuscular injections of Aroclor 1254 (a polychlorinated biphenyl) produced significant increases in APDM and AH activities (P less than or equal to 0.1, ANOVA). There were no significant changes in the metabolism of toluene, liver wet weights, or liver protein concentrations following treatment.

摘要

研究了与杂种太阳鱼（蓝鳃太阳鱼×绿太阳鱼×驼背太阳鱼）肝脏匀浆12,000g上清液部分相关的酶对挥发性芳烃甲苯的体外代谢。测定了氨基比林脱甲基酶（APDM）和苯胺羟化酶（AH）的活性。肌肉注射多氯联苯Aroclor 1254后，APDM和AH活性显著增加（P≤0.1，方差分析）。处理后甲苯代谢、肝脏湿重或肝脏蛋白质浓度均无显著变化。