Melito I, Prado W A, Corrado A P, Netto J C
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1984;78(1):171-3. doi: 10.1016/0742-8413(84)90065-3.
Tityustoxin (TsTx), a toxic fraction of Tityus serrulatus venom, was studied on the isolated guinea-pig vas deferens. It increased significantly the maximal response of the preparation to both norepinephrine and acetylcholine and decreased the effective median dose of norepinephrine. The effect of TsTx on norepinephrine median dose was unchanged when atropinized or pharmacologically "denervated" preparations were used but was abolished when both procedures were associated. Atropinization of pharmacologically denervated muscles almost never modify the TsTx-induced increase in the maximal response to norepinephrine. On denervated or phentolamine-treated muscles TsTx-induced increase in the maximal response to acetylcholine was abolished. It was concluded that toxin predominantly induces adrenergic postsynaptic supersensitivity. Of minor significance, it also induces presynaptic cholinergic and adrenergic supersensitivity. Comparison of these results with those of crude venom indicates that TsTx effects may result from the sum of the effects of subcomponents not demonstrated by the chemical procedures here utilized.
锯齿蝎毒素（TsTx）是锯齿蝎毒液的一种毒性成分，我们对其在分离的豚鼠输精管上进行了研究。它显著增加了标本对去甲肾上腺素和乙酰胆碱的最大反应，并降低了去甲肾上腺素的有效中剂量。当使用阿托品化或药理学上“去神经”的标本时，TsTx对去甲肾上腺素中剂量的作用没有改变，但当两种处理同时进行时，该作用被消除。药理学上去神经的肌肉进行阿托品化几乎不会改变TsTx诱导的对去甲肾上腺素最大反应的增加。在去神经或用酚妥拉明处理的肌肉上，TsTx诱导的对乙酰胆碱最大反应的增加被消除。得出的结论是，该毒素主要诱导肾上腺素能突触后超敏反应。次要的是，它还诱导突触前胆碱能和肾上腺素能超敏反应。将这些结果与粗毒液的结果进行比较表明，TsTx的作用可能是此处所采用化学方法未证实的亚成分作用之和。
