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表皮生长因子抑制A431人癌细胞中核蛋白细胞周期蛋白的合成。

Epidermal growth factor inhibits the synthesis of the nuclear protein cyclin in A431 human carcinoma cells.

作者信息

Bravo R

出版信息

Proc Natl Acad Sci U S A. 1984 Aug;81(15):4848-50. doi: 10.1073/pnas.81.15.4848.

DOI:10.1073/pnas.81.15.4848
PMID:6146981
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC391588/
Abstract

The effect of epidermal growth factor (EGF) on the synthesis of the nuclear protein cyclin and its relationship with cell proliferation has been studied in human carcinoma A431 cells. Quantitative two-dimensional gel electrophoretic analysis of [35S]methionine-labeled polypeptides from EGF-treated and untreated A431 cells showed that the synthesis of cyclin (0.07% of total labeled protein) could be decreased to 20% (less than 0.01% of total labeled protein) by EGF at a concentration of 100 ng/ml. A good correlation between the effect of different concentrations of EGF on cyclin synthesis and A431 cell proliferation was found. A431-derived cells resistant to the growth-inhibitory effect of EGF showed no significant changes in cyclin synthesis after EGF treatment. Taken together, these results support the idea that cyclin may be an important protein that is involved in the control of cell proliferation.

摘要

在人A431癌细胞中研究了表皮生长因子（EGF）对核蛋白细胞周期蛋白合成的影响及其与细胞增殖的关系。对经EGF处理和未经处理的A431细胞中[35S]甲硫氨酸标记的多肽进行定量二维凝胶电泳分析，结果显示，在浓度为100 ng/ml的EGF作用下，细胞周期蛋白的合成（占总标记蛋白的0.07%）可降至20%（低于总标记蛋白的0.01%）。发现不同浓度的EGF对细胞周期蛋白合成的影响与A431细胞增殖之间存在良好的相关性。对EGF生长抑制作用具有抗性的A431衍生细胞在EGF处理后细胞周期蛋白合成未显示出显著变化。综上所述，这些结果支持细胞周期蛋白可能是参与细胞增殖控制的一种重要蛋白质这一观点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ad/391588/64343bc23154/pnas00616-0240-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ad/391588/c3123aa78e6c/pnas00616-0240-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ad/391588/d4ee4f3cb371/pnas00616-0240-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ad/391588/64343bc23154/pnas00616-0240-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ad/391588/c3123aa78e6c/pnas00616-0240-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ad/391588/d4ee4f3cb371/pnas00616-0240-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02ad/391588/64343bc23154/pnas00616-0240-c.jpg

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Epidermal growth factor inhibits the synthesis of the nuclear protein cyclin in A431 human carcinoma cells.表皮生长因子抑制A431人癌细胞中核蛋白细胞周期蛋白的合成。
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8
Epidermal growth factor-induced stimulation of epidermal growth factor-receptor synthesis in human cytotrophoblasts and A431 carcinoma cells.表皮生长因子诱导人细胞滋养层细胞和A431癌细胞中表皮生长因子受体合成的刺激作用。
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本文引用的文献

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Identification of a nuclear and of a cytoplasmic polypeptide whose relative proportions are sensitive to changes in the rate of cell proliferation.一种细胞核和细胞质多肽的鉴定，其相对比例对细胞增殖速率的变化敏感。
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[35S]-methionine labelled polypeptides from secondary mouse kidney fibroblasts: coordinates and one dimensional peptide maps of some major polypeptides.来自次级小鼠肾成纤维细胞的[35S] - 甲硫氨酸标记的多肽：一些主要多肽的坐标和一维肽图。
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Distribution of HeLa cells polypeptides in cytoplasts and karyoplasts.
Stimulation and inhibition of growth by EGF in different A431 cell clones is accompanied by the rapid induction of c-fos and c-myc proto-oncogenes.
在不同的A431细胞克隆中，表皮生长因子（EGF）对生长的刺激和抑制伴随着原癌基因c-fos和c-myc的快速诱导。
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An enzymatic assay reveals that proteins destined for the apical or basolateral domains of an epithelial cell line share the same late Golgi compartments.一项酶活性测定表明，上皮细胞系顶侧或基底外侧区域的目标蛋白共用相同的晚期高尔基体区室。
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Existence of two populations of cyclin/proliferating cell nuclear antigen during the cell cycle: association with DNA replication sites.细胞周期中细胞周期蛋白/增殖细胞核抗原的两种群体的存在：与DNA复制位点的关联。
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Cloning and sequence of the human nuclear protein cyclin: homology with DNA-binding proteins.人类核蛋白细胞周期蛋白的克隆与序列分析：与DNA结合蛋白的同源性
Proc Natl Acad Sci U S A. 1987 Mar;84(6):1575-9. doi: 10.1073/pnas.84.6.1575.
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Cell cycle-dependent variations in the distribution of the nuclear protein cyclin proliferating cell nuclear antigen in cultured cells: subdivision of S phase.培养细胞中核蛋白细胞周期蛋白增殖细胞核抗原分布的细胞周期依赖性变化：S期的细分
Proc Natl Acad Sci U S A. 1985 May;82(10):3262-6. doi: 10.1073/pnas.82.10.3262.
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A restricted set of apical proteins recycle through the trans-Golgi network in MDCK cells.在MDCK细胞中，一组受限的顶端蛋白通过反式高尔基体网络进行循环利用。
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Two independent mechanisms for escaping epidermal growth factor-mediated growth inhibition in epidermal growth factor receptor-hyperproducing human tumor cells.在表皮生长因子受体高表达的人类肿瘤细胞中，存在两种独立的机制来逃避表皮生长因子介导的生长抑制。
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海拉细胞多肽在胞质体和核质体中的分布。
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Architecture and polypeptide composition of HeLa cytoskeletons. Modification of cytoarchitectural polypeptides during mitosis.HeLa细胞骨架的结构与多肽组成。有丝分裂过程中细胞结构多肽的修饰。
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Epidermal growth factor inhibits growth of A431 human epidermoid carcinoma in serum-free cell culture.表皮生长因子在无血清细胞培养中抑制A431人表皮样癌的生长。
J Cell Biol. 1982 Apr;93(1):1-4. doi: 10.1083/jcb.93.1.1.
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A search for differential polypeptide synthesis throughout the cell cycle of HeLa cells.对整个海拉细胞周期中差异多肽合成的研究。
J Cell Biol. 1980 Mar;84(3):795-802. doi: 10.1083/jcb.84.3.795.
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Analysis of morphology and receptor metabolism in clonal variant A431 cells with differing growth responses to epidermal growth factor.对表皮生长因子生长反应不同的克隆变体A431细胞的形态学和受体代谢分析。
J Cell Physiol. 1983 Jun;115(3):235-42. doi: 10.1002/jcp.1041150304.
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Role of epidermal growth factor-stimulated protein kinase in control of proliferation of A431 cells.表皮生长因子刺激的蛋白激酶在A431细胞增殖调控中的作用。
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Altered epidermal growth factor (EGF)-stimulated protein kinase activity in variant A431 cells with altered growth responses to EGF.在对表皮生长因子（EGF）生长反应改变的A431变异细胞中，EGF刺激的蛋白激酶活性发生改变。
Proc Natl Acad Sci U S A. 1982 Apr;79(8):2574-8. doi: 10.1073/pnas.79.8.2574.
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Human proteins sensitive to neoplastic transformation in cultured epithelial and fibroblast cells.在培养的上皮细胞和成纤维细胞中对肿瘤转化敏感的人类蛋白质。
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