Groothuis G M, Hulstaert C E, Kalicharan D, Hardonk M J
Virchows Arch B Cell Pathol Incl Mol Pathol. 1984;46(1-2):77-82. doi: 10.1007/BF02890297.
Administration of N-hydroxy-2-acetylaminofluorene (90 mumol/kg, iv) to rats results in damage to periportal hepatocytes. The most prominent ultrastructural changes are the appearance of numerous unusual vesicles of about 200 nm diameter and the proliferation of the endoplasmic reticulum to form fingerprint-like structures. In order to characterize these vesicles and to investigate their possible origin, their enzymatic activity was studied by ultrastructural enzyme histochemistry. The vesicles as well as the fingerprint-like structures exhibited glucose-6-phosphatase activity. Continuities between the vesicles and the membranes of both the RER and the SER were frequently seen. The vesicles lacked ATPase, 5'nucleotidase and catalase activity. From these results we conclude that the vesicles may be an unusual proliferation of the endoplasmic reticulum.
给大鼠静脉注射N-羟基-2-乙酰氨基芴（90微摩尔/千克）会导致肝门周围肝细胞受损。最显著的超微结构变化是出现大量直径约200纳米的异常小泡以及内质网增殖形成指纹状结构。为了表征这些小泡并研究其可能的来源，通过超微结构酶组织化学研究了它们的酶活性。这些小泡以及指纹状结构均表现出葡萄糖-6-磷酸酶活性。经常可见小泡与粗面内质网和滑面内质网的膜之间存在连续性。这些小泡缺乏ATP酶、5'-核苷酸酶和过氧化氢酶活性。根据这些结果，我们得出结论，这些小泡可能是内质网的一种异常增殖。
