Flaks B, Yost Y, Flaks A
Carcinogenesis. 1982;3(1):103-9. doi: 10.1093/carcin/3.1.103.
Inbred male Leeds strain rats were given a diet containing 0.05% N-fluoren-4-ylacetohydroxamic acid for up to 7 months. A modified method for the synthesis of this compound is described. Rats were killed at intervals during and after treatment and their liver tissues examined by light and electron microscopy. The treated rats developed a very low incidence of (non-hepatic) tumours and hepatic clear cell foci appeared during the late stages of the experiment. The only significant fine structural changes observed were glycogenosis and alterations in the morphology of the rough endoplasmic reticulum, both of which developed only after prolonged treatment and affected a minority of hepatocytes. These changes are discussed in relation to the fine structural changes elicited by the strongly carcinogenic N-fluoren-2-ylacetamide and the non-carcinogen, N-fluoren-4-ylacetamide.
将近交系雄性利兹品系大鼠喂养含0.05% N-芴-4-基乙酰氧肟酸的饲料长达7个月。描述了该化合物的一种改良合成方法。在治疗期间及之后定期处死大鼠，并通过光镜和电镜检查其肝脏组织。经处理的大鼠发生（非肝脏）肿瘤的发生率极低，且在实验后期出现了肝脏透明细胞灶。观察到的唯一显著的细微结构变化是糖原沉积和粗面内质网形态改变，这两种变化均仅在长期治疗后出现，且仅影响少数肝细胞。结合强致癌性的N-芴-2-基乙酰胺和非致癌物N-芴-4-基乙酰胺引发的细微结构变化对这些变化进行了讨论。
