Neylan D, Smyth P P
Cell Biochem Funct. 1983 Jul;1(2):112-6. doi: 10.1002/cbf.290010213.
Lysosomal membrane permeability was assessed by measuring freely available naphthylamidase activity in intact preparations of guinea pig thyroid follicular cells following exposure of thyroid tissue to sequential stimulation by two thyroid stimulators, thyrotrophin (TSH) and thyroid stimulating immunoglobulins (TSI). These investigations showed that following labilization by TSH, the lysosomal membranes recovered and were capable of responding to a second thyroid stimulator (TSI). That such recovery represented restabilization of lysosomal membranes was confirmed by the finding that latent naphthylamidase activity was restored without a change in total activity of the enzyme.
通过测量豚鼠甲状腺滤泡细胞完整制剂中可自由利用的萘基酰胺酶活性，评估溶酶体膜通透性。甲状腺组织先后受到两种甲状腺刺激物，即促甲状腺激素（TSH）和甲状腺刺激免疫球蛋白（TSI）刺激后进行此项研究。这些研究表明，经TSH使溶酶体膜不稳定后，其能够恢复并对第二种甲状腺刺激物（TSI）作出反应。潜伏性萘基酰胺酶活性得以恢复而酶的总活性无变化，这一发现证实了这种恢复代表溶酶体膜的再稳定。
