Ealey P A, Smyth P P
J Immunoassay. 1980;1(2):175-94. doi: 10.1080/01971528008055783.
A cytochemical bioassay for thyroid stimulators which utilised 12 micrometer sections of guinea pig thyroid as the target tissue was validated and applied to the measurement of thyroid stimulating antibodies (T.S.Ab.) in human plasma. T.S.H. and T.S.Ab. could be distinguished in the assay by the times at which they produced maximal responses; 90 seconds for T.S.H. and 180 seconds for T.S.Ab. Homogeneity of responses to stimulation was relatively uniform throughout thyroid tissue enabling simultaneous measurement of several plasma samples per guinea pig. Sensitivity of the assay was 1.5 x 10(-9) microU/l MRC L.A.T.S.-B. Antiserum to H-T.S.H. produced no significant change in T.S.Ab.-like activity when added to plasma from a thyrotoxic patient. Both anti-IgG and aliquots of a homogenate of thyroid tissue from a thyrotoxic patient diminished responses of thyrotoxic plasma in a concentration dependent manner. T.S.Ab. was present at titres of 1/100 to 1/100,00 in 13 hyperthyroid patients tested to date.
一种利用豚鼠甲状腺12微米切片作为靶组织的甲状腺刺激物细胞化学生物测定法得到验证，并应用于测定人血浆中的甲状腺刺激抗体（T.S.Ab.）。在该测定法中，促甲状腺激素（T.S.H.）和甲状腺刺激抗体可通过它们产生最大反应的时间来区分；促甲状腺激素为90秒，甲状腺刺激抗体为180秒。整个甲状腺组织对刺激的反应均匀性相对一致，使得每只豚鼠能够同时测定多个血浆样本。该测定法的灵敏度为1.5×10⁻⁹微单位/升MRC L.A.T.S.-B。抗H-T.S.H.抗血清添加到甲状腺毒症患者血浆中时，甲状腺刺激抗体样活性无显著变化。抗IgG和来自甲状腺毒症患者的甲状腺组织匀浆等分试样均以浓度依赖方式降低甲状腺毒症血浆的反应。在迄今为止检测的13例甲状腺功能亢进患者中，甲状腺刺激抗体的滴度为1/100至1/100,00。
