Nassar C F, Jurjus A R, Haddad M E, Sarru E
Comp Biochem Physiol A Comp Physiol. 1984;79(1):161-4. doi: 10.1016/0300-9629(84)90725-4.
Procaine inhibited significantly (P less than 0.01) alanine accumulation in the rat intestinal strips in a concentration-dependent pattern, whereas it showed no effect on alanine uptake by the turtle intestinal cells. Colchicine and Vinca alkaloids at 5 X 10(-4) and 1.5 X 10(-6) M respectively caused a significant inhibition (P less than 0.01) of intracellular alanine concentration in the rat with no effect noticed in the turtle. Unidirectional influx of alanine across the brush border membrane of the rat was significantly (P less than 0.01) reduced in the presence of procaine, colchicine and vincristine in the preincubation medium. The same drugs did not show any effect on alanine influx into the turtle small intestine. Electron microscopy showed major structural alterations in the cytoskeletal organization of the turtle intestine in response to procaine, colchicine or vincristine treatment. It is proposed that microtubular system may participate in the overall transport mechanism of alanine across the small intestine.
普鲁卡因能显著抑制（P<0.01）大鼠肠段中丙氨酸的积累，且呈浓度依赖性，而对龟肠细胞摄取丙氨酸无影响。秋水仙碱和长春花生物碱分别在5×10⁻⁴和1.5×10⁻⁶M时可显著抑制（P<0.01）大鼠细胞内丙氨酸浓度，对龟则无此作用。在预孵育培养基中存在普鲁卡因、秋水仙碱和长春新碱时，丙氨酸跨大鼠刷状缘膜的单向内流显著减少（P<0.01）。相同药物对丙氨酸流入龟小肠无任何影响。电子显微镜显示，经普鲁卡因、秋水仙碱或长春新碱处理后，龟肠的细胞骨架组织发生了主要结构改变。推测微管系统可能参与了丙氨酸跨小肠的整体转运机制。
