Penner M, Coon C N
Poult Sci. 1980 May;59(5):1109-13. doi: 10.3382/ps.0591109.
Asparaginase and asparagine synthetase specific activity (enzyme units/mg protein) was determined in chicks and rats fed various protein diets. The specific activity of asparaginase was twofold higher in the kidney compared to the liver of chicks fed a 25% protein control diet, whereas the specific activity of asparaginase was eightfold greater in the liver compared to kidney tissue of rats fed the 25% protein control diet. The asparaginase specific activity in the chick liver, chick kidney, and rat liver were all significantly increased when animals were fed the 75% protein diet. Asparaginase specific activity increased significantly in rat and chick kidneys when the animals were fasted. Dietary supplementation of asparagine did not significantly increase asparaginase specific activity in either animal. The combined kidney and liver asparagine synthetase specific activity for chicks fed the control diets is over seven hundred times less than the corresponding combined asparaginase specific activity. Rats fed the control diet had a combined kidney and liver asparagine synthetase specific activity about 75 times less than the corresponding asparaginase specific activity. The asparagine synthetase specific activity was significantly increased in the rat and chick kidney when a 2% protein diet was fed.
测定了喂食不同蛋白质饮食的雏鸡和大鼠体内天冬酰胺酶和天冬酰胺合成酶的比活性（酶单位/毫克蛋白质）。与喂食25%蛋白质对照饮食的雏鸡肝脏相比，其肾脏中天冬酰胺酶的比活性高出两倍；而与喂食25%蛋白质对照饮食的大鼠肾脏组织相比，其肝脏中天冬酰胺酶的比活性高出八倍。当动物喂食75%蛋白质饮食时，雏鸡肝脏、雏鸡肾脏和大鼠肝脏中的天冬酰胺酶比活性均显著增加。当动物禁食时，大鼠和雏鸡肾脏中的天冬酰胺酶比活性显著增加。饮食中添加天冬酰胺对两种动物的天冬酰胺酶比活性均无显著增加。喂食对照饮食的雏鸡，其肾脏和肝脏中天冬酰胺合成酶的联合比活性比相应的天冬酰胺酶联合比活性低七百多倍。喂食对照饮食的大鼠，其肾脏和肝脏中天冬酰胺合成酶的联合比活性比相应的天冬酰胺酶比活性低约75倍。当喂食2%蛋白质饮食时，大鼠和雏鸡肾脏中天冬酰胺合成酶的比活性显著增加。
