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通过酪氨酸羟化酶免疫细胞化学法证实的七种物种视网膜中假定的含多巴胺细胞。

Putative dopamine-containing cells in the retina of seven species demonstrated by tyrosine hydroxylase immunocytochemistry.

作者信息

Ballesta J, Terenghi G, Thibault J, Polak J M

出版信息

Neuroscience. 1984 Aug;12(4):1147-56. doi: 10.1016/0306-4522(84)90009-5.

DOI:10.1016/0306-4522(84)90009-5
PMID:6148714
Abstract

Immunocytochemistry with antibodies to catecholamine synthesizing enzymes has revealed cells in the retina of chick, mouse, hamster, rat, guinea-pig, piglet and marmoset which contain tyrosine hydroxylase but not dopamine beta-hydroxylase. These findings suggest that the cells in question produce dopamine but that catecholamine synthesis does not proceed further to noradrenaline. Tyrosine hydroxylase-containing amacrine cells, located in the innermost part of the inner nuclear layer, were present in all the species studied. Some species showed atypically located amacrine cells in the inner plexiform or ganglion cell layer. In the rodents, the existence of tyrosine hydroxylase-containing interplexiform cells was suggested by the presence of a few short immunoreactive ascending processes. Three different morphological types of putative dopamine-containing cells were classified according to the level of ramification.

摘要

用针对儿茶酚胺合成酶的抗体进行免疫细胞化学研究发现,在鸡、小鼠、仓鼠、大鼠、豚鼠、仔猪和狨猴的视网膜中存在一些细胞,这些细胞含有酪氨酸羟化酶,但不含有多巴胺β-羟化酶。这些发现表明,所讨论的细胞产生多巴胺,但儿茶酚胺的合成不会进一步转化为去甲肾上腺素。含酪氨酸羟化酶的无长突细胞位于内核层的最内层,在所研究的所有物种中均有发现。一些物种在内网状层或神经节细胞层中显示出非典型定位的无长突细胞。在啮齿动物中,通过少数短的免疫反应性上升突起的存在,提示存在含酪氨酸羟化酶的网间细胞。根据分支水平,将三种不同形态类型的假定含多巴胺细胞进行了分类。

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