Wagemann R, Hunt R, Klaverkamp J F
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1984;78(2):301-7. doi: 10.1016/0742-8413(84)90087-2.
The subcellular distribution of Zn, Cd, Cu and Hg in liver and kidney from a narwhal was determined by ultracentrifugation and gel filtration. Most of the total mercury in the liver and kidney was bound by the cellular pellet (88 and 73%, respectively). Of the total mercury, 7 and 11% was in the form of methylmercury in the liver and kidney, respectively. More than half (74%) of the total Zn and Cu in the kidney was in the cytosol and somewhat less than this was in the cytosol of the liver. Almost all of the cadmium in liver and kidney (88 and 92%, respectively) was in cytosol. Cytosolic fractions from liver and kidney were evaluated for the presence of metallothionein by analysing for Zn, Cd, Hg, Cu, Fe and--SH groups, by molecular weight estimation and by u.v. absorption spectra. Metallothionein was found in these organs in estimated concentrations similar to those present in terrestrial and other marine mammals.
通过超速离心和凝胶过滤法测定了独角鲸肝脏和肾脏中锌、镉、铜和汞的亚细胞分布。肝脏和肾脏中总汞的大部分与细胞沉淀相结合（分别为88%和73%）。肝脏和肾脏中总汞分别有7%和11%以甲基汞的形式存在。肾脏中总锌和总铜的一半以上（74%）存在于胞质溶胶中，肝脏胞质溶胶中的这一比例略低于此值。肝脏和肾脏中几乎所有的镉（分别为88%和92%）都存在于胞质溶胶中。通过分析锌、镉、汞、铜、铁和巯基基团、估计分子量以及紫外吸收光谱，对肝脏和肾脏的胞质溶胶部分进行了金属硫蛋白的检测。在这些器官中发现的金属硫蛋白估计浓度与陆地和其他海洋哺乳动物体内的浓度相似。
