Tunnacliffe A, Crawford L V, Goodfellow P
Mol Cell Biol. 1984 Aug;4(8):1657-60. doi: 10.1128/mcb.4.8.1657-1660.1984.
Previous work has shown that murine embryonal carcinoma cells are refractory to infection with various viruses, including simian virus 40. Thus, large T and small t antigens, the products of the simian virus 40 early region, are not produced when the virus infects embryonal carcinoma cells, in contrast to other cell types. We show, by qualitative and quantitative analyses, that embryonal carcinoma cell hybrids, containing a simian virus 40 early region integrated into human DNA, are capable of producing viral large T antigen.
先前的研究表明，鼠胚胎癌细胞对包括猿猴病毒40在内的多种病毒感染具有抗性。因此，与其他细胞类型不同，当猿猴病毒40感染胚胎癌细胞时，不会产生该病毒早期区域的产物——大T抗原和小t抗原。我们通过定性和定量分析表明，含有整合到人类DNA中的猿猴病毒40早期区域的胚胎癌细胞杂种能够产生病毒大T抗原。
