Azoury R, Sarig S, Garti N, Perlberg S, Randolph A D, Drach G W
Urol Res. 1984;12(4):223-6. doi: 10.1007/BF00256808.
Crystal Size Distribution (CSD) and the yield of Calcium Oxalate Crystals in solutions with an admixture of 5 normal and 3 stone forming urines, were determined. A positive correlation was found between the median size, the number of particles and the overall inhibitory potentials of the urines toward calcium oxalate precipitation in vitro as reflected by Discriminating Index (DI) measurements. Incubation of two samples of stone formers' (SF) urines with glutamic-oxalacetic-transaminase (GOT) caused a reduction of aspartic acid concentration, an increase in glutamic acid concentration and a parallel decrease in the DI values. After 90 min of SF urine incubation with GOT the DI in three samples was improved and both the median size and number of particles reduced, by 28% and 45% respectively. These results could indicate that GOT activity changes the inhibitory power of the SF urine by transforming aspartic acid into glutamic acid, having thus most probably a part in the inhibition of CaOx stone formation.
测定了含有5份正常尿液和3份结石形成尿液混合物的溶液中草酸钙晶体的晶体尺寸分布（CSD）和产率。通过鉴别指数（DI）测量发现，尿液的中位尺寸、颗粒数量与尿液对草酸钙沉淀的总体抑制潜力之间存在正相关。将两份结石形成者（SF）的尿液样本与谷草转氨酶（GOT）一起孵育，导致天冬氨酸浓度降低、谷氨酸浓度升高，同时DI值平行下降。在SF尿液与GOT孵育90分钟后，三个样本中的DI得到改善，中位尺寸和颗粒数量分别减少了28%和45%。这些结果可能表明，GOT活性通过将天冬氨酸转化为谷氨酸来改变SF尿液的抑制能力，因此很可能在抑制草酸钙结石形成中起作用。
