Drach G W, Thorson S, Randolph A
J Urol. 1980 Apr;123(4):519-23. doi: 10.1016/s0022-5347(17)56001-2.
The urine of normal persons, as well as that of stone formers, contains inhibitors to crystallization of calcium oxalate. To ascertain differences, if any, between these 2 groups we measured nucleation rate, growth rate and total mass produced in an analytic system with 5 per cent urine added to 95 per cent synthetic urine that did not contain large organic molecules. We also observed the effects of addition of uromucoid to the synthetic solution. Normal urine and stone-forming urine contained significant inhibitors to total mass of calcium oxalate dihydrate crystals precipitated but total crystal mass was not significantly different between the 2 groups. Growth rate was significantly less in experiments with the urine of stone formers. The other major difference found in stone-forming urine was significant enhancement of nucleation rate when compared to normal urine. Since addition of uromucoid produced these same growth inhibition and nucleation enhancement effects but did not affect total crystal mass, we hypothesize that uromucoid has an important part in producing the differences noted between and stone-forming urine.
正常人的尿液以及结石形成者的尿液中都含有草酸钙结晶抑制剂。为了确定这两组之间是否存在差异，我们在一个分析系统中测量了成核速率、生长速率和产生的总质量，该系统是向95%不含大有机分子的合成尿液中添加5%的尿液。我们还观察了向合成溶液中添加尿黏蛋白的效果。正常尿液和结石形成者的尿液中都含有对二水合草酸钙晶体沉淀总质量的显著抑制剂，但两组之间的总晶体质量没有显著差异。结石形成者尿液的实验中生长速率明显较低。在结石形成者的尿液中发现的另一个主要差异是，与正常尿液相比，成核速率显著提高。由于添加尿黏蛋白产生了相同的生长抑制和成核增强作用，但不影响总晶体质量，我们推测尿黏蛋白在产生正常尿液和结石形成者尿液之间的差异方面起重要作用。
