Corradetti R, Lo Conte G, Moroni F, Passani M B, Pepeu G
Eur J Pharmacol. 1984 Sep 3;104(1-2):19-26. doi: 10.1016/0014-2999(84)90364-9.
The effect of adenosine and related compounds on the release of endogenous aspartate and glutamate from isolated, superfused rat hippocampal slices was studied at rest and during electrical stimulation of the stratum radiatum in the CA3/CA2 region, using a sensitive mass-spectrometric technique. Evoked extracellular potentials were recorded from the CA1 region. Adenosine, at 3 X 10(-4) M concentration, inhibited the stimulation-evoked potentials and prevented the stimulation-induced release of aspartate and glutamate. Similarly, 1-phenylisopropyladenosine (10(-6) M) and cyclohexyladenosine (10(-6) M) depressed both electrical and neurochemical responses to stimulation of the stratum radiatum. 8-Phenyltheophylline (5 X 10(-6) M) increased the release of aspartate and glutamate and antagonized the cyclohexyladenosine-induced inhibition of amino acid release. Our results support the hypothesis that adenosine modulates the electrophysiological responses to stimulation of stratum radiatum through a reduction of the release of the excitatory amino acids aspartate and glutamate.
运用灵敏的质谱技术，研究了腺苷及相关化合物对分离的、经灌流的大鼠海马切片中内源性天冬氨酸和谷氨酸释放的影响，实验分别在静息状态以及对CA3/CA2区辐射层进行电刺激时进行。从CA1区记录诱发的细胞外电位。浓度为3×10⁻⁴M的腺苷抑制刺激诱发的电位，并阻止刺激诱导的天冬氨酸和谷氨酸释放。同样，1-苯异丙基腺苷（10⁻⁶M）和环己基腺苷（10⁻⁶M）抑制对辐射层刺激的电反应和神经化学反应。8-苯甲基茶碱（5×10⁻⁶M）增加天冬氨酸和谷氨酸的释放，并拮抗环己基腺苷诱导的氨基酸释放抑制。我们的结果支持这样的假说，即腺苷通过减少兴奋性氨基酸天冬氨酸和谷氨酸的释放来调节对辐射层刺激的电生理反应。
