Oudega B, Mooi F R, de Graaf F K
Antonie Van Leeuwenhoek. 1984;50(5-6):569-84. doi: 10.1007/BF02386227.
In gram-negative bacteria only few proteins are exported across both the cytoplasmic membrane and the outer membrane which forms an extra barrier for protein excretion. In this review we describe the mechanisms of production and export of two types of plasmid-encoded proteins in Escherichia coli. These proteins are the bacteriocin cloacin DF13 and the K88ab and K99 fimbrial subunits. Specific so-called helper proteins located at different positions in the cell envelope play an essential role in the export of these proteins. The genetic organization, subcellular location and functions of these helper proteins, as well as the effects of mutations and culture conditions on the export of the proteins are described. Models for the export mechanisms are presented and future application possibilities for engineering foreign protein excretion in E. coli with these export systems are discussed.
在革兰氏阴性菌中，只有少数蛋白质能够穿过细胞质膜和外膜，而外膜对蛋白质分泌形成了额外的屏障。在这篇综述中，我们描述了大肠杆菌中两种质粒编码蛋白质的产生和分泌机制。这些蛋白质是细菌素cloacin DF13以及K88ab和K99菌毛亚基。位于细胞膜不同位置的特定所谓辅助蛋白在这些蛋白质的分泌中起着至关重要的作用。描述了这些辅助蛋白的基因组织、亚细胞定位和功能，以及突变和培养条件对蛋白质分泌的影响。提出了分泌机制的模型，并讨论了利用这些分泌系统在大肠杆菌中工程化外源蛋白质分泌的未来应用可能性。
