Kan E A, Siskind G W
J Immunol. 1980 Apr;124(4):1758-62.
The appearance of degenerate antibody-secreting cells early in the course of the immune response was demonstrated. Mice immunized with SRBC had anti-SRBC plaques that were as much as 95% inhibitable by DNP-Ova or DNP-KLH, and mice immunized with DNP-KLH had anti-DNP plaques that were as much as 90% inhibitable by Fl-BGG. In addition, mice immunized with BRBC produced plaques detectable on TNP-SRBC. The data suggest that, early after antigen injection, antibody is produced that can react with both the immunizing antigen and with structurally unrelated antigenic determinants. The incidence of such degenerate antibody-secreting cells is maximal at 3 days after antigen injection and decreases thereafter. It is probable that the degenerate antibody is of very low affinity for the immunizing antigen.
在免疫反应过程早期出现了退化的抗体分泌细胞，这一点得到了证实。用绵羊红细胞（SRBC）免疫的小鼠产生的抗SRBC噬斑，高达95%可被二硝基苯卵清蛋白（DNP - Ova）或二硝基苯钥孔戚血蓝蛋白（DNP - KLH）抑制；用DNP - KLH免疫的小鼠产生的抗DNP噬斑，高达90%可被荧光标记的牛γ球蛋白（Fl - BGG）抑制。此外，用溴化红细胞（BRBC）免疫的小鼠产生的噬斑可在三硝基苯绵羊红细胞（TNP - SRBC）上检测到。这些数据表明，在注射抗原后早期，产生的抗体既能与免疫抗原反应，也能与结构不相关的抗原决定簇反应。这种退化的抗体分泌细胞的发生率在抗原注射后3天最高，之后下降。很可能这种退化抗体对免疫抗原的亲和力非常低。
