Doria G, Mancini C, DeLisi C
J Immunol. 1978 Nov;121(5):2030-4.
The hemolytic plaque inhibition assay has been performed on spleen cells from mice immunized with TNP-HRBC to evaluate avidity of anti-TNP IgM antibodies. At different times after immunization direct plaques were inhibited by soluble TNP-EACA, TNP61-BGG, or anti-mu antiserum. Analysis of the inhibition data provided independent estimates of antibody avidity and secretion rate. Avidity was found to increase with time, to reach a maximum when the antibody response attained the peak value, and then to decline as the response was waning. There was a decrease followed by increase of the secretion rate concomitant with the rise and fall of the antibody response and avidity.
已对用TNP-人红细胞（TNP-HRBC）免疫的小鼠脾细胞进行溶血空斑抑制试验，以评估抗TNP IgM抗体的亲和力。免疫后不同时间，可溶性TNP-EACA、TNP61-牛γ球蛋白（TNP61-BGG）或抗μ抗血清可抑制直接空斑。对抑制数据的分析提供了抗体亲和力和分泌率的独立估计值。发现亲和力随时间增加，在抗体反应达到峰值时达到最大值，然后随着反应减弱而下降。分泌率随着抗体反应和亲和力的上升和下降而先降低后升高。
