Baird L G, Tachovsky T G, Sandberg-Wollheim M, Koprowski H, Nisonoff A
J Immunol. 1980 May;124(5):2324-8.
Anti-idiotypic antibodies were prepared in mouse ascites fluid against the CSF-IgG of a patient with multiple sclerosis. After adsorption with pooled human IgG, the ascites fluid antibodies precipitated 20% of labeled autologous CSF IgG. By using a competitive radioimmunoassay, less than one microgram of unlabeled CSF IgG produced 50% inhibition of binding autologous 125I-labeled CSF IgG, whereas 50 micrograms of normal HIgG was not inhibitory. The idiotype could be found in both serum and CSF IgG and persisted over a 5-year period. The absolute concentration of idiotype in the CSF varied somewhat but remained from 4 to 10 times greater than that of the serum. One of 14 heterologous MS CSF was found to contain small amounts of inhibitory protein; eight CSF from patients with other neurologic diseases did not contain the idiotype.
以一名多发性硬化症患者的脑脊液免疫球蛋白（CSF-IgG）为抗原，在小鼠腹水中制备了抗独特型抗体。用混合人免疫球蛋白吸附后，腹水抗体沉淀了20%的标记自体脑脊液免疫球蛋白。通过竞争性放射免疫测定法，不到1微克的未标记脑脊液免疫球蛋白可产生50%的自体125I标记脑脊液免疫球蛋白结合抑制，而50微克的正常人类免疫球蛋白则无抑制作用。独特型可在血清和脑脊液免疫球蛋白中发现，并持续存在5年。脑脊液中独特型的绝对浓度有所变化，但仍比血清中的高4至10倍。14份异源性多发性硬化症脑脊液中有1份含有少量抑制性蛋白；8份其他神经系统疾病患者的脑脊液中未含有独特型。
