Fridman W H, Gresser I, Bandu M T, Aguet M, Neauport-Sautes C
J Immunol. 1980 May;124(5):2436-41.
Murine T2D4 cells derived from a T cell hybrid line were incubated with partially purified or electrophoretically pure mouse interferon and tested for the expression of Fc gamma R as assessed by a) counting the number of cells forming rosettes with IgG-sensitized sheep erythrocytes, and b) incubating the cells with heat-aggregated rabbit IgG and then determining either the number of cells stained with fluorescein conjugated goat anti-rabbit IgG or the extent of labeling by using radioactive iodinated staphylococcus protein A. Although interferon induced a rapid increase in Fc gamma R expression on the Fc gamma R-positive T2D4 cells, it did not induce either Fc gamma R on the Fc gamma R negative BW5147 cells or Fc gamma R on either cell line. Human leukocyte interferon enhanced the expression of Fc gamma R on human Burkitt cells (Daudi) but did not affect the expression of Fc gamma R on mouse cells. We suggest that interferon may influence several effector functions of the immune system by modulating Fc receptor expression.
将源自T细胞杂交系的小鼠T2D4细胞与部分纯化或经电泳纯化的小鼠干扰素一起孵育，通过以下方法检测FcγR的表达：a）计算与IgG致敏的绵羊红细胞形成玫瑰花结的细胞数量；b）将细胞与热聚集的兔IgG孵育，然后确定用荧光素偶联的山羊抗兔IgG染色的细胞数量，或使用放射性碘化葡萄球菌蛋白A确定标记程度。虽然干扰素可使FcγR阳性的T2D4细胞上的FcγR表达迅速增加，但它既不能诱导FcγR阴性的BW5147细胞上的FcγR表达，也不能诱导这两种细胞系中的任何一种表达FcγR。人白细胞干扰素可增强人伯基特细胞（Daudi）上FcγR的表达，但不影响小鼠细胞上FcγR的表达。我们认为，干扰素可能通过调节Fc受体的表达来影响免疫系统的几种效应功能。
