Effects of PGI2 and PGI analogues on cAMP levels in cultured endothelial and smooth muscle cells derived from bovine arteries.

PGI2 increased cAMP levels in cultured endothelial cells derived from bovine aortas in the presence of isobutylmethylxanthine (IBMX). Stable PGI analogues were more effective, while PGE1 was without effect. In cultured smooth muscle cells from bovine aortas, PGI2, pGE1 and the stable PGI analogues dose-dependently increased cAMP levels in the absence and presence of IBMX. The data confirm the stimulatory effect of PGI2 on cAMP content in bovine coronary artery rings and suggest that the effect is mainly due to the stimulatory action of PGI2 on cAMP in smooth muscle cells.