Dickson A J, Pogson C I
Biochem J. 1980 Jan 15;186(1):35-45. doi: 10.1042/bj1860035.
Methods have been derived which permit the isolation of undergraded polyribosomes from isolated rat liver cells. Under the conditions used the polyribosome profile of hepatocytes immediately after isolation was essentially identical with that from intact liver. However, during incubation of cells in complex physiological media there was a progressive dissociation of polyribosomes. The addition of a variety of factors that produce reaggregation of polyribosomes in rat liver in vivo did not prevent dissociation during cell incubations. Although large polyribosomes were lost most rapidly, the albumin-synthesizing capacity of isolated cells was not selectively lost when compared with total protein synthesis. The significance of these results for the use of isolated hepatocytes in the study of liver protein synthesis is discussed.
已开发出一些方法，可从分离的大鼠肝细胞中分离出未降解的多核糖体。在所使用的条件下，分离后肝细胞的多核糖体图谱与完整肝脏中的基本相同。然而，在复杂生理培养基中培养细胞时，多核糖体逐渐解离。添加多种在体内能使大鼠肝脏中的多核糖体重新聚集的因子，并不能阻止细胞培养过程中的解离。尽管大的多核糖体丢失得最快，但与总蛋白质合成相比，分离细胞的白蛋白合成能力并未选择性丧失。本文讨论了这些结果对于在肝脏蛋白质合成研究中使用分离肝细胞的意义。
