Tamura T, Chiba S, Abo W, Chiba Y, Nakao T
Infect Immun. 1980 Apr;28(1):49-53. doi: 10.1128/iai.28.1.49-53.1980.
The role of specific cell-mediated immune responses in human cytomegalovirus (CMV) infections was studied by an in vitro lymphocyte transformation test, using a whole blood culture. CMV-induced in vitro lymphocyte proliferation was mainly dependent on the presence of sensitized T-cells. Three of five seronegative patients with B-cell deficiency showed positive lymphocyte responses to CMV antigen. In contrast, a patient with ataxia-telangiectasia who was shedding the virus in urine did not show a cellular response to either CMV or phytohemagglutinin. Age-related differences were found in the CMV lymphocyte transformation test. The responses were generally low (P less than 0.025) in infants and higher in older children and adults. CMV lymphocyte transformation responses were lower in 15 children who were excreting CMV in urine when tested than in 17 seropositive children who were not excreting the virus.
通过全血培养的体外淋巴细胞转化试验，研究了特异性细胞介导的免疫反应在人巨细胞病毒（CMV）感染中的作用。CMV诱导的体外淋巴细胞增殖主要依赖于致敏T细胞的存在。五名B细胞缺陷的血清阴性患者中有三名对CMV抗原表现出阳性淋巴细胞反应。相比之下，一名在尿液中排出病毒的共济失调毛细血管扩张症患者对CMV或植物血凝素均未表现出细胞反应。在CMV淋巴细胞转化试验中发现了与年龄相关的差异。婴儿的反应通常较低（P小于0.025），而大龄儿童和成人的反应较高。在检测时，15名尿液中排出CMV的儿童的CMV淋巴细胞转化反应低于17名未排出病毒的血清阳性儿童。
