反式氧化茋对大鼠肝细胞溶质中谷胱甘肽S-转移酶A、B和C的诱导作用。

Induction of glutathione S-transferases A, B and C in rat liver cytosol by trans-stilbene oxide.

作者信息

Guthenberg C, Morgenstern R, DePierre J W, Mannervik B

出版信息

Biochim Biophys Acta. 1980 Aug 1;631(1):1-10. doi: 10.1016/0304-4165(80)90047-1.

Abstract

The cytoplasmic glutathione S-transferase activity of rat liver has been shown to increase to 300--400% of control values after treatment of the animals with trans-stilbene oxide and this phenomenon has been further characterized in the present study. Quantitative immunological determinations showed that the content of glutathione S-transferases A, B and C together constituted 4.5% of the soluble proteins in the hepatic cytoplasm of untreated rats. The content rose to 12.9 and 17.4% after treatment with trans-stilbene oxide or a combination of trans-stilbene oxide, 3-methylcholanthrene and phenobarbital, respectively. It was demonstrated that the cytosolic fraction from induced liver contains 4.2 times as much antigen which can be precipitated with antiglutathione S-transferase B antiserum as does control cytosol. Antiglutathione S-transferase C, which intereacts with transferases A and C, precipitates 3.3 times as much protein from the induced cytosol compared with control. Crossed immunoelectrophoresis and purification demonstrated that both A and C are increased in amount after treatment with trans-stilbene oxide. Thus, cytosolic glutathione S-transferases A, B, and C in liver are all induced by treatment of rats with trans-stilbene oxide. Immunological crossreaction, similar behavior during chromatography on CM-cellulose and hydroxyapatite and similar specific activities suggest that the control and induced enzymes are essentially identical, trans-Stilbene oxide was found to serve as a relatively poor second substrate for glutathione S-transferases A, B and C and can thus be said to cause substrate induction of these enzymes.

摘要

用反式氧化 stilbene 处理大鼠后，已显示大鼠肝脏的细胞质谷胱甘肽 S - 转移酶活性增加至对照值的300 - 400%，并且在本研究中对这一现象进行了进一步表征。定量免疫测定表明，谷胱甘肽 S - 转移酶A、B和C的含量一起占未处理大鼠肝细胞质中可溶性蛋白质的4.5%。用反式氧化 stilbene 或反式氧化 stilbene、3 - 甲基胆蒽和苯巴比妥的组合处理后，含量分别升至12.9%和17.4%。已证明，诱导肝脏的胞质部分含有比对照胞质溶胶多4.2倍的可被抗谷胱甘肽 S - 转移酶B抗血清沉淀的抗原。与转移酶A和C相互作用的抗谷胱甘肽 S - 转移酶C，与对照相比，从诱导胞质溶胶中沉淀的蛋白质多3.3倍。交叉免疫电泳和纯化表明，用反式氧化 stilbene 处理后，A和C的量均增加。因此，用反式氧化 stilbene 处理大鼠可诱导肝脏中的胞质谷胱甘肽 S - 转移酶A、B和C。免疫交叉反应、在CM - 纤维素和羟基磷灰石上色谱时的相似行为以及相似的比活性表明，对照酶和诱导酶基本相同。反式氧化 stilbene 被发现是谷胱甘肽 S - 转移酶A、B和C相对较差的第二底物，因此可以说它导致这些酶的底物诱导。