α2成分对血清中妊娠特异性β1糖蛋白免疫测定的干扰。

Interference of an alpha 2 component in immunological determinations of pregnancy-specific beta 1 glycoprotein in serum.

作者信息

Pala A, Di Ruzza A, Curtilli L, Strom R, Moro M, Reverberi L, Carenza L

机构信息

Institute of 2nd Clinics of Obstetrics and Gynaecology, University of Rome, La Sapienza, Italy.

出版信息

Clin Chim Acta. 1987 Sep 15;168(1):33-46. doi: 10.1016/0009-8981(87)90264-6.

DOI:10.1016/0009-8981(87)90264-6

PMID:3499261

Abstract

Pregnancy-specific beta 1 glycoprotein (SP1-beta) was purified from human retroplacental blood by sequential anion exchange chromatography, gel chromatography and affinity chromatography. The final preparation appeared to be electrophoretically and immunochemically pure and was in particular free from any component with alpha mobility. The preparation was used as immunogen in rabbits as well as tracer and standard for radioimmunoassay and for cross- and rocket-immunoelectrophoresis. It was shown that this radioimmunoassay procedure, allowed quantitative determination of SP1-beta glycoprotein without interference by the alpha component.

摘要

通过连续阴离子交换色谱、凝胶色谱和亲和色谱从人胎盘后血液中纯化出妊娠特异性β1糖蛋白（SP1-β）。最终制品在电泳和免疫化学上显示为纯品，尤其不含任何具有α迁移率的成分。该制品用作兔的免疫原以及放射免疫测定、交叉免疫电泳和火箭免疫电泳的示踪剂与标准品。结果表明，这种放射免疫测定方法能够定量测定SP1-β糖蛋白，而不受α成分的干扰。