妊娠特异性β1糖蛋白的分子异质性：对放射免疫测定和免疫电泳测定的影响。

Molecular heterogeneity of pregnancy specific beta 1 glycoprotein: the effect on measurement by radioimmunoassay and electroimmunoassay.

作者信息

Teisner B, Grudzinskas J G, Hindersson P, Al-Ani A T, Westergaard J G, Chard T

出版信息

J Immunol Methods. 1979;31(1-2):141-9. doi: 10.1016/0022-1759(79)90294-1.

Abstract

A comparison of methods for the quantification of circulating pregnancy specific beta 1 glycoprotein in late pregnancy was performed to assess the influence of the presence of a high molecular weight glycoprotein with alpha 2 electrophoretic mobility (SP1 alpha) which is immunochemically identifiable with pregnancy-specific PBETA 1 glycoprotein (SP1 beta). Serum samples from 47 volunteers in the 3rd trimester of pregnancy were subjected to measurements of pregnancy specific beta 1 glycoprotein in rocket immunoelectrophoresis, quantitative crossed-immunoelectrophoresis and radioimmunoassay. Rocket immunoelectrophoresis gives a result which reflects the total SP1 content, i.e. SP1 alpha and SP1 beta while quantitative crossed-immunoelectrophoresis permits differentiation between the two molecules. Radioimmunoassay predominantly measures authenic SP1, i.e. SP1 beta in the presence of physiological amounts of SP1 alpha.

摘要

为评估具有α2电泳迁移率的高分子量糖蛋白（SP1α）的存在对妊娠晚期循环妊娠特异性β1糖蛋白定量方法的影响，进行了一项比较研究，该高分子量糖蛋白在免疫化学上可与妊娠特异性PBETA 1糖蛋白（SP1β）识别。对47名妊娠晚期志愿者的血清样本进行火箭免疫电泳、定量交叉免疫电泳和放射免疫测定，以检测妊娠特异性β1糖蛋白。火箭免疫电泳给出的结果反映了总SP1含量，即SP1α和SP1β，而定量交叉免疫电泳可区分这两种分子。放射免疫测定主要测量真实的SP1，即在生理量的SP1α存在下的SP1β。