Fumagalli R, Bernini F, Galli G, Anastasia M, Fiecchi A
Steroids. 1980 Jun;35(6):665-72. doi: 10.1016/0039-128x(80)90091-4.
Treatment of pregnant rats with AY-9944, a drug interfering with the last steps of cholesterol biosynthesis, accumulated cholesterol precursors in brain and liver of newborn animals. Different sterol profiles were found in these organs. Along with cholesterol and cholesta-5,7-dien-3 beta-ol, present in both tissues, liver was found to contain a hitherto unreported sterol, absent in brain. The structure of cholesta-5,8-dien-3 beta-ol was attributed to this compound by mass spectrometric, 1H, and 13C NMR analysis.
用AY - 9944（一种干扰胆固醇生物合成最后步骤的药物）处理怀孕大鼠，会使新生动物的脑和肝脏中积累胆固醇前体。在这些器官中发现了不同的甾醇谱。除了在两种组织中都存在的胆固醇和胆甾 - 5,7 - 二烯 - 3β - 醇外，还发现肝脏中含有一种迄今未报道的甾醇，而脑内不存在这种甾醇。通过质谱、¹H和¹³C NMR分析，将这种化合物的结构确定为胆甾 - 5,8 - 二烯 - 3β - 醇。
