防腐剂对鸡胚气管纤毛摆动频率的影响。

The effects of preservatives on the ciliary beat frequency of chicken embryo tracheas.

作者信息

van de Donk H J, Muller-Plantema I P, Zuidema J, Merkus F W

出版信息

Rhinology. 1980 Sep;18(3):119-33.

Abstract

The effects of preservatives on the ciliary beat frequency of chicken embryo tracheas are determined, Polar compounds like benzalkonium chloride in commonly used concentrations, decrease the frequency less than 30% after a 20 minutes' exposure. The effect is not reversible after rinsing with Locke-Ringer solution. Lipophilic compounds however, like chlorbutol, cause an arrest of the cilary movement with 10 minutes. The effect, different from the polar compounds, is reversible; but only after a limited exposure-time. Mercuric compounds, like thiomersal, decrease the frequency non-reversibly 30 to 90% after a 20 minutes' exposure. EDTA decreases the frequency 40 to 50%, independent of the exposure-time and in a reversible way. The combination of benzalkonium chloride 0.01% and EDTA 0.05% is recommended to preserve nasal drops.

摘要

测定了防腐剂对鸡胚气管纤毛摆动频率的影响。常用浓度的极性化合物如苯扎氯铵，暴露20分钟后频率降低不到30%。用洛克-林格溶液冲洗后，这种影响是不可逆的。然而，亲脂性化合物如三氯叔丁醇，10分钟内会导致纤毛运动停止。与极性化合物不同，这种影响是可逆的，但仅在有限的暴露时间后。汞化合物如硫柳汞，暴露20分钟后频率不可逆地降低30%至90%。乙二胺四乙酸（EDTA）可降低频率40%至50%，与暴露时间无关且是可逆的。建议将0.01%的苯扎氯铵和0.05%的EDTA组合用于滴鼻剂的保存。