van de Donk H J, Muller-Plantema I P, Zuidema J, Merkus F W
Rhinology. 1980 Sep;18(3):119-33.
The effects of preservatives on the ciliary beat frequency of chicken embryo tracheas are determined, Polar compounds like benzalkonium chloride in commonly used concentrations, decrease the frequency less than 30% after a 20 minutes' exposure. The effect is not reversible after rinsing with Locke-Ringer solution. Lipophilic compounds however, like chlorbutol, cause an arrest of the cilary movement with 10 minutes. The effect, different from the polar compounds, is reversible; but only after a limited exposure-time. Mercuric compounds, like thiomersal, decrease the frequency non-reversibly 30 to 90% after a 20 minutes' exposure. EDTA decreases the frequency 40 to 50%, independent of the exposure-time and in a reversible way. The combination of benzalkonium chloride 0.01% and EDTA 0.05% is recommended to preserve nasal drops.
测定了防腐剂对鸡胚气管纤毛摆动频率的影响。常用浓度的极性化合物如苯扎氯铵，暴露20分钟后频率降低不到30%。用洛克-林格溶液冲洗后，这种影响是不可逆的。然而，亲脂性化合物如三氯叔丁醇，10分钟内会导致纤毛运动停止。与极性化合物不同，这种影响是可逆的，但仅在有限的暴露时间后。汞化合物如硫柳汞，暴露20分钟后频率不可逆地降低30%至90%。乙二胺四乙酸（EDTA）可降低频率40%至50%，与暴露时间无关且是可逆的。建议将0.01%的苯扎氯铵和0.05%的EDTA组合用于滴鼻剂的保存。
