Guarente L, Roberts T M, Ptashne M
Science. 1980 Sep 19;209(4463):1428-30. doi: 10.1126/science.6158095.
Methods are described that allow efficient expression in Escherichia coli of cloned eukaryotic genes. The methods require that the coding sequence of the gene in question be available in a form uninterrupted by intervening sequences (for example, as a complementary DNA clone). The gene products are synthesized unfused to other amino acid sequences. The genetic manipulations are simple, and require the plasmids described and commercially available enzymes.
本文描述了一些方法，可使克隆的真核基因在大肠杆菌中高效表达。这些方法要求所研究基因的编码序列以不被间隔序列打断的形式存在（例如，作为互补DNA克隆）。基因产物在合成时不与其他氨基酸序列融合。基因操作简单，并且需要所述质粒和市售酶。
